Move Exchange 2010 Trasport Queue

My recommendation will be to use the script as it takes care of the steps needed.

• The script will create the folder if it doesn’t already exist.
• It will give the necessary accounts the correct permissions on the folder. ( Full control for Network Service, System & Administrators).
• It will move the transport database (Mail.Que) and log files to the new drive.
• It will restart the transport service for the changes to take effect.
• Editing the config files doesn’t move the transport database & log files. It just updates Exchange to use the new drive once the service is restarted.

The command to move the transport queue is the same in both 2007 & 2010.
./Move-TransportDatabase.ps1 –QueueDatabasepath “path” –QueueDatabaseLoggingPath “path”

Reference:
http://www.howexchangeworks.com/2010/08/moving-transport-queue-in-exchange-2010.html